When considering anti-squat:

1. It's best to use the sprocket combination in which you care most about the pedaling properties. For a winch-and-plummet bike, that could be a large cassette sprocket; for a DH race bike, it's probably the most commonly used sprinting sprocket.

2. Ensure variables are as consistent as possible: centre of mass, sag, sprockets, etc. Even if they're wrong, such as the centre of mass, you can still do good design work if you're consistent!